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Environmental Biotechnology Research Group
 Group Leader:
 
 
 Current Members

PhD Students: Ms Aneela Iqbal, Ms. Iram Gul, Ms. Maria Manzoor, Ms. Zahra
MS Students: Mr. Maaz Ahmad, Ms. Sana Nisar, Ms. Shagufta Irum, Ms. Hira Imam Jamil, Ms. Ayesha, Ms. Fariha Ajmal, Ms. Farhat Yasmeen, Ms. Syeda Phyzia Tariq, Ms. Anum Amin, Ms. Saima, Ms. Farhat Yasmeen. 

Introduction
 
Environmental Biotechnology Research Group, formed in Jan 2011 at IESE, NUST is the pioneer group in this field under the supervision of Dr. Muhammad Arshad. The aim of this group is to identify, characterize and develop indigenous solutions to challenging environmental problems through biotechnological approaches. The group is involved in performing research on various aspects of plant responses to environmental stresses including physiology, nutrition, mechanisms, soil environment, heavy metals toxicity, antibiotics toxicity, nanoparticles application, microbial and bacterial degradation of toxic compounds, bioremediation, phytoremediation and ethanol production from cellulose, etc. Efforts are underway to develop strategies and standardize experimental protocols, explore new opportunities for innovation and to contribute in the development of society. 

Activities
 
EBG is involved in cutting-edge research activities in phytoavailability of nutrients using nanotechnology approach. The group is also investigating the role & application of microbial consortium to induce phtoavailability of heavy metals and associated plant responses (including tolerance, hyper accumulation and toxicity) along with application of soil amendments (organic/ inorganic) to facilitate the soil remediation process. Because of the fact that these abiotic stresses affect crop yield and food security and, have health concerns. Current research uses a suite of molecular biology tools and plant physiology studies to determine how plants and microorganisms together can contribute to solve the problem of heavy metal contamination. Major research activities include:
 
Investigating the effects of nanoparticles on different plant species
Isolation of metal resistant and plant growth promoting bacteria and fungi    
Functional and molecular characterization of microorganisms
Treatment of heavy metal contaminated soil through biological and chemical means
Investigating the impact of antibiotics on crop yields and strategies to mitigate the effects in order to ensure food security
Antibiotic resistance in microbes present in environmental samples

Students learn a variety of research methodologies including but not limited to chemical analysis, quantitative analysis, statistical analysis, biomolecular methods, bioinformatics tools and data collection in the laboratory and the field assays to conduct research in their specific sub-fields. Researchers make full use of their abilities in diverse fields not only in academia and research labs but also in public organizations, industries, central and regional governments and NGO’s. 

Research Projects/ Grants
a) 2017-2020: Enhancing Biogas Production from Sewage Sludge and Ligno-Cellulosic Waste through Nanoparticles Assisted Chemical Pretreatment. Funded by Higher Education Commission of Pakistan. PKR 4.344 million. Co-PI
b) 2016-2019: Ensuring Food Security and Safety: Development of an Integrated Approach for Safe Usage of Antibiotics Containing Wastewater for Irrigation of Crop Plants. Funded by Higher Education Commission of Pakistan. PKR 6.034 million. Principal Investigator. 
c) 2015-2018: Soil–applied TiO2 nanoparticles affect the phytoavailability of phosphorus and yield of rice and wheat. Funded by Higher Education Commission of Pakistan. PKR 2.911 million. Principal Investigator. 
d) 2015-2018: Development of an integrated strategy for phytoremediation of lead contaminated soils. PHC-PERIDOT, Jointly funded by Pak-France Governments. PKR 1.45 million. Principal Investigator. 
e) 2013-2014: Recovery of cellulose from cotton gin waste and conversion to Carboxymethyl Cellulose (CMC) along with toxicity and economic analysis. Funded by Industry – Reso-Chem, Lahore, Pakistan. PKR 0.5 million. Co–Principal Investigator.
f) 2011-2012: Comet assay setup for environmental toxicity studies at IESE-SCEE-NUST Islamabad. Funded by Higher Education Commission of Pakistan. PKR 0.5 million. Principal Investigator.

Research Supervision

MS Environmental Science Students – Completed

1. Ms. Marium Fiaz. 2017. Screening of microbes isolated from environmental samples for antibiotics resistance.
2. Ms. Neelam Naqvi. 2016. Effect of DIPA and EDTA on lead phytoavailability and uptake. 
3. Ms. Anza Javaid. 2016. Isolation and identification of chromium tolerant bacterial strains and their potential to promote plant growth
4. Ms. Rubab Zahra. 2016. Phytotoxicity in response to NPs in Rice and their impacts on Animal Model
5. Ms. Naima Waseem. 2016. Nutrient availability and gene expression response to nanoparticles in rice
6. Mr. Muhammad Bilal. 2016. Cadmium accumulation and its effect on physiological activity and oil production in aquatic macrophytes
7. Mr. Salman Ahmed. 2016. Kinetics of benzene degradation using selected bacterial strains 
8. Ms. Komal Siddique. 2016.  Phytoavailability of nitrogen in response to application of titania nanoparticles in soil
9. Ms. Ayesha Neelam. 2015. Synthesis of a carboxymethyl cellulose (CMC) based composite membrane and testing its performance in saline water treatment
10. Ms. Bushra. 2016. Lipids production from oleaginous microbes using agricultural residues as a feedstock   
11. Mr. Muhammad Saad Bin Zahid. 2015. Benzene degradation using Rhizospheric Bacteria
12. Ms. Rafia Rafique. 2014. Physiological effects of TiO2 nanoparticles on wheat and lettuce plants in the context of phosphorus bioavailability
13. Ms. Maha Zafar. 2014. Determination of the genotoxic potential of particulate matter in human blood cells
14. Ms. Nahdia Samreen. 2014. Toxicological effects of pure and doped titania nano-particles on leukocytes
15. Ms. Hina Imtiaz. 2014. Impacts of ciprofloxacin on plant growth and soil microbial biomass
16. Ms. Zahra Zahra. 2014. Phytoavailability of nutrients in response to different nanoparticles application in soil.
17. Ms. Rafia Khalid. 2014. Cadmium removal from contaminated water using aquatic floating macrophyte - Nasturtium officinale.
18. Mr. Sana Ullah. 2013. Risk assessment of TiO2 nanoparticles using crop plants: Exposure response of wheat.
19. Ms. Samreen Akhtar. 2013. Toxicity assessment of ciprofloxacin on Triticum aestivum. 
20. Ms. Saddaf Rashid. 2012. Assessing genotoxic effects of airborne pollutants in leucocytes. 
21. Ms. Maryam Siddiqa. 2012. Genotoxicity of pesticides in industry workers.
22. Ms. Hina Umber Hanif. 2012. Phytoavailability of phosphorous affected by TiO2 nanoparticles. 

PhD Environmental Science Students – in progress

1. Ms Aneela Iqbal. Phytoremediation of petrochemicals from wastewater using endophytic bacteria
2. Ms. Iram Gul. Organic and Inorganic amendments mediated phytoextraction of lead and cadmium 
3. Ms. Maria Manzoor. Microbiological interventions at soil-plant interface to induce lead mobility and its remediation
4. Ms. Zahra. Mobility and uptake of phosphorus by rice and wheat in tio2 nanoparticles amended soil

MS Environmental Science Students – in progress

1. Mr. Maaz Ahmad. Effects of soil pH on phosphorous availability and microbial population upon TiO2 nanoparticles application 
2. Ms. Sana Nisar. Impact of soil texture on growth of rice and    multi-nutrient availability in titania amended     soils
3. Ms. Hira Imam Jamil. Growth response and toxicity induced in wheat irrigated with antibiotic containing water
4. Ms. Shagufta Irum. Comparative analysis of fertilizer levels and application methods of titania nanoparticles on the growth of rice (oryza sativa l.)
5. Ms. Ayesha. Role of organic matter and fertilizers in alleviation of antibiotic toxicity in plants.
6. Ms. Fariha Ajmal. Integrated approach for phytoremediation of lead contaminated soil using Pelargonium species.
7. Ms. Anum Amin. Degradation of antibiotics by nanoparticles and its impact on wheat growth
8. Ms. Sayyad Phyzia Tariq. Toxicity of TiO 2 NPs on Rice Plant and Soil Microorganisms
9. Ms. Saima. Detection of Antibiotic Resistance Genes (ARGs) in antibiotics resistant phenotypes. 
10. Ms. Farhat Yasmeen. Role of different levels of fertilizers in alleviation of toxicity in rice crop.

Environmental Engineering Undergraduate Students – Final Year Project

1. Hamna Aamir, Iqra Javed, Wajeeha Naz. 2015. Cellulose isolation from cotton gin waste and synthesis of cellulose/PVA membrane for heavy metals removal. Declared as the best project among peers.
2. Muhammad Ali Inam, Qudsia Khanam, Syed Asar Ul Hasnain, Talha Bin Umeed. 2014. Production of cellulose acetate from biowaste.
3. Adnan Maqbool, Maseeh-ud-Din, Saliha Akram, Syed Iftikhar Hussain, Warda Ajaz. 2012. Color removal from textile wastewater using biosorbents.

Journal Publications

1. Zahra Z, Waseem N, Zahra R, Lee H, Badshah MA, Mehmood A, Choi H-K, Arshad M*. 2017. Growth and metabolic responses of rice (Oryza sativa L.) cultivated in phosphorus-deficient soil amended with TiO2 nanoparticles. J Agric Food Chem. 65(28):5598-5606. IF 3.154
2. Iqbal A, Arshad M*, Hashmi H, Karthikeyan R, Gentry TJ, Schwab, AP. 2017. Biodegradation of phenol and benzene by endophytic bacterial strains isolated from refinery wastewater-fed Cannabis sativa. Environ Technol, DOI: 10.1080/09593330.2017.1337232, IF 1.715
3. Hina K, Arshad M, Latif H, Ghafoor A, Rafique H. 2017. Sorption of metals by tree bark from synthetic contaminated water. Pak J Agri Sci, DOI: 10.21162/PAKJAS/17.5864, IF 0.609
4. Arshad M, Merlina G, Uzu G, Sobanska S, Sarret G, Dumat C, Silvestre J, Pinelli E, Kallerhoff J. 2016. Phytoavailability of lead altered by two Pelargonium cultivars grown on contrasting lead-spiked soils. J Soils Sediments, 16, 581-591, IF 2.522
5. Zahid MS, Iqbal A, Arshad M*. 2016. Benzene degradation with bacterial strains isolated from rhizosphere of Cannabis sativa being irrigated with petroleum refinery wastewater. Desalin Water Treat, 57, 17579-84, IF 1.631
6. Arshad M*, Siddiqa M, Rashid S, Hashmi I, Awan MA, Ali MA. 2016. Biomonitoring of toxic effects of pesticides in occupationally exposed individuals. Saf Health Work. 7, 156-160.
7. Mehmood CT, Qazi IA, Baig MA, Arshad M, Quddos A. 2016. Enhanced photodegradation of titania loaded polyethylene films in a humid environment. Int Biodeter Biodegr, 113, 287–296, IF 2.962
8. Mustafa G, Ali MA, Smith D, Schwinghamer T, Lamont JR, Ahmed N, Hussain S, Arshad M. 2016. Guar, jantar, wheat straw, and rice hull composts as replacements for peat in muskmelon transplant production. Int J Recycl Org Waste Agricult, 5:323–33
9. Khan AHA, Anees M, Arshad M, Muhammad YS, Iqbal M, Yousaf S. 2016. Effects of illuminance and nutrients on bacterial photo-physiology of hydrocarbon degradation. Sci Total Environ, 557–558, 705–711, IF 4.900
10. Husnain A, Qazi IA, Khaliq W, Arshad M. 2016. Immobilization in cement mortar of chromium removed from water using titania nanoparticles. J Environ Manage, 172, 10-17, IF 4.010
11. Imran B, Khan SJ, Qazi IA, Arshad M. 2016. Removal and recovery of sodium hydroxide (NaOH) from industrial wastewater by two-stage diffusion dialysis (DD) and electrodialysis (ED) processes. Desalin Water Treat, 57, 7926–7932, IF 1.631 
12. Waheed H, Hashmi I, Khan SJ, Kim SR, Arshad M, Nasir H. 2016. Microbial population dynamics and profiling of quorum sensing agents in membrane bioreactor. Int Biodeter Biodegr, 113, 66–73, IF 2.962 
13. Ali SS, Qazi IA, Arshad M, Khan Z, Voice TC, Mehmood CT. 2016. Photocatalytic degradation of low density polyethylene (LDPE) films using titania nanotubes. Environ Nanotech Monitor Manage, 5, 44–53.
14. Mehmood CT, Arshad M*, Ashfaq T, Bilal M, Shafiq M, Hina K. 2016. Kinetics of adsorptive removal of Drimarene Brilliant Red from aqueous solution using untreated agricultural residues. Pak J Sci Ind Res Ser. A: Phys. Sci. 59, 11-22.
15. Zafar M, Arshad M*, Khokhar MF. 2015. Assessment of particulate matter (PM10) and polycyclic aromatic hydrocarbons levels at various sites in Faisalabad and their potential toxicity. Pak J Agri Sci, 52, 233-238, IF 0.597
16. Zahra Z, Arshad M*, Rafique R, Mahmood A, Habib A, Qazi IA, Khan SA. 2015. Metallic Nanoparticle (TiO2 and Fe3O4) application modifies rhizosphere phosphorus availability and uptake by Lactuca sativa. J Agric Food Chem. 63, 6876-6882, IF 2.857
17. Hanif HU, Arshad M*, Ali MA, Ahmed N, Qazi IA. 2015. Phytoavailability of phosphorus to Lactuca sativa in response to soil applied TiO2 nanoparticles. Pak J Agri Sci, 52, 177-182, IF 0.597
18. Mehmood CM, Qazi IA, Baig MA, Arshad M, Quddos A. 2015. Application of photodegraded polythene films for the treatment of Drimarene Brilliant Red (DBR) dye. Int Biodeter Biodegr, 102, 31-39, IF 2.429
19. Abbas J, Khokhar MF, Arshad M. 2015.  Monitoring formaldehyde concentration over Islamabad using ground based and satellite observations. J Space Tech, 5, 83-90.
20. Arshad M*, Khalid R, Hina K, Ullah S, Ali MA. 2015. Cadmium removal by aquatic macrophyte (Nasturtium officinale) and potential for oil production from the biomass. Pak J Agri Agril Engg Vet Sci, 31, 1-13
21. Latif W, Qazi IA, Hashmi I, Arshad M, Nasir H, Habib A. 2014. Novel method for preparation of pure and iron doped Titania nanotube coated wood surfaces to disinfect airborne bacterial species Pseudomonas aeruginosa and Staphylococcus aureus. Env Sci Eng, 31, 681-688. IF 0.991
22. Haleem N, Arshad M*, Shahid M, Tahir MA. 2014. Synthesis of carboxymethyl cellulose from waste of cotton ginning industry. Carbohyd Polym, 113, 249–255. IF 4.074
23. Shahid M, Austruy A, Echevarria G, Arshad M, Sanaullah M, Aslam M, Nadeem M, Nasim W, Dumat C. 2014. EDTA-enhanced phytoremediation of heavy metals: A review. Soil Sediment Contam, 23, 389–416. IF 1.039
24. Rafique R, Arshad M*, Khokhar MF, Qazi IA, Hamza A, Virk N. 2014. Growth response of wheat to Titania nanoparticles application. NUST J Engg Sci, 7, 42-46
25. Ullah S, Arshad M*. 2014. Exposure-response of Triticum aestivum to Titanium dioxide nanoparticles application: Seedling Vigor Index and micronuclei formation. Sci Vision, 20, 57-61
26. Rafique A, Awan MA, Wasti A, Qazi IA, Arshad M. 2013. Removal of fluoride from drinking water using modified immobilized activated alumina. J Chem, 2013, 7 pages, ID 386476, http://dx.doi.org/10.1155/2013/386476. IF 0.622
27. Arshad M, Silvestre J, Merlina G, Dumat C, Pinelli E, Kallerhoff J. 2012. Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars. Plant Cell Tiss Organ Cult, 108, 315-322. IF 3.633
28. Shahid M, Arshad M, Kaemmerer M, Pinelli E, Probst A, Baque D, Pradere P, Dumat C. 2012. Long-term field metal extraction by Pelargonium: Phytoextraction efficiency in relation to plant maturity.  Int J Phytorem, 14, 493-505. IF 1.179
29. Arshad M*, Murtaza G, Ali MA, Shafiq M, Dumat C, Ahmed N. 2011. Wheat growth and phytoavailability of copper and zinc as affected by soil texture in saline-sodic conditions. Pak J Bot, 43, 2433-2439. IF 0.907
30. Arshad M, Silvestre J, Pinelli E, Kallerhoff J, Kaemmerer M, Tarigo A, Shahid M, Guiresse M, Pradere  P,  Dumat C. 2008. A field study of lead phytoextraction by various scented Pelargonium cultivars. Chemosphere, 71, 2187-2192. IF 3.054
31. Arshad M, Silvestre J, Kallerhoff J, Dumat C. 2007. Des géraniums odorants pour décontaminer les sols. Info Chimie, 482, 62-65.

Conference Papers

1. Arshad M, Neelam A, Haleem N. 2017. Preparation and characterization of bio-based composite membrane for water production. 7th International Conference Environmentally Sustainable Development (ESDev-VII) on 26-28 August, COMSATA, Abbottabad, Pakistan.  
2. Gul I, Manzoor M, Kallerhoff J, Arshad M. 2017. Phytoremediation ability of Pelargonium zonale in cadmium contaminated soil. 7th International Conference Environmentally Sustainable Development (ESDev-VII) on 26-28 August, COMSATA, Abbottabad, Pakistan.  
3. Ahmad M, Arshad M. 2017. Growth response of Triticum aestivum to TiO2 nanoparticles. 7th International Conference Environmentally Sustainable Development (ESDev-VII) on 26-28 August, COMSATA, Abbottabad, Pakistan. 
4. Jamil HM, Arshad M. 2017. Phytotoxicity of antibiotics to seed germination and biomass in Triticum aestivum. 2017.  7th International Conference Environmentally Sustainable Development (ESDev-VII) on 26-28 August, COMSATA, Abbottabad, Pakistan. 
5. Manzoor M, Gul I, Kallerhoff J, Arshad M, 2017. Evaluating potential of ornamental plants to grow in lead contaminated soil. 7th International Conference Environmentally Sustainable Development (ESDev-VII) on 26-28 August, COMSATA, Abbottabad, Pakistan.
6. Arshad M, Akhtar S, Imtiaz H, Zahra Z, Khan SA. 2017. Early growth responses of Triticum aestivum to ciprofloxacin and DNA damage assessments through comet assay. 210th International Conference on Environmental Science and Development, Seoul, South Korea 18-19 Jul 2017.
7. Arshad M, Zahra Z, Rafique R, Zahra R, Waseem N. 2017. Nanoparticles Application to Soil: Nutrient Availability, Plant Growth, Toxicity and Food Chain Contamination, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
8. Kallerhoff J, Arshad M, 2017. Phytotechnologies for Environmental Clean-Up, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
9. Manzoor M, Gul I, Arshad M, Kallerhoff J, Hashmi I, Khan MZA, 2017. Screening of Native Ornamental Species as Phytoremediation Technology for Pb Contaminated Soil; Species Variation in Lead Uptake and Translocation, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
10. Gul I, Manzoor M, Arshad M, Hashmi I, Khokhar F, Virk NM, Kallerhoff J, 2017. Potential of Different Organic and Inorganic Amendments in Increasing Bioavailability of Pb and Cd in Soil, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
11. Iqbal A, Arshad M, Karthikeyan R, Schwab AP, Gentry TJ, 2017, Phytoremediation of Phenol in Hypersaline Conditions by Endophytic Bacillus sp. B2, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
12. Kanwal S, Baig S, Hashmi I, Arshad M, 2017. Growth Response in Two Main Tree Species of Islamabad Under Drought Stress and Nitrogen Supply, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
13. Batool A, Zeshan, Arshad M, Khan SJ, 2017. Effect of Substrates on Phytoremediation of Trace Metals from Solid Waste Leachate, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
14. Nisar S, Arshad M, Zeeshan, Bhatti F, 2017. Impact of Soil Texture on Growth of Rice (Oryza sativa L.) in Titania Amended Soils, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
15. Fiaz M, Ahmed I, Hashmi I, Manzoor M, Gul I, Arshad M, 2017. Isolation of Multiples Antibiotic Resistant Microbes from Pharmaceutical Wastewater, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
16. Arshad M, Manzoor M, Gul I, Kallerhoff J, 2017. Plant Growth Promotion in Heavy Metal Contaminated Soil through Multiple Heavy Metal Resistant Bacteria, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
17. Zahra Z, Waseem N, Zahra R, Arshad M, Choi HK, Parveen A, Baig S, Khokhar MF, 2017. Early Growth Response of Wheat (Triticum aestivum) Cultivars to Titanium Dioxide (tio2) Nanoparticles, International Conference on Bioapproaches for Environment and Sustainability (ICBES) on 22-23 February 2017 Islamabad, Pakistan.
18. Zahra, Arshad M. 2014. Synthesis Methods of Titania and Magnetite Nanoparticles for Application to Crop Plants”, 3rd ASEAN-Pakistan Conference on Materials Science (APCoMs 3) on 25-27 November 2014 Islamabad, Pakistan
19. Rafique R, Arshad M. 2014. Growth response of wheat to Titania nanoparticles application. 3rd ASEAN-Pakistan Conference on Materials Science (APCoMs 3) on 25-27 November 2014 Islamabad, Pakistan
20. Arshad M, Siddiqa M, Hashmi I, Awan MA, Ali MA. 2014. DNA damage assessments in blood samples from workers exposed to pesticides in industrial area. 7th International Conference on Challenges in Environmental Science & Engineering, Johar Bahru, Malaysia. 12-16 Oct. 2014.
21. Zahid MS, Iqbal A, Arshad M. 2014. Isolation and identification of rhizospheric bacteria for the degradation of Benzene. 15th International Congress of Soil Science on “Soil Management in Changing Climate”, Islamabad, Pakistan. 18-20 Mar, 2014.
22. Arshad M, Zahra Z. 2014. Nanotechnology in agriculture. A Pakistani perspective. Advancements in Science and Research, CIIT Vehari, Pakistan. 17th Mar, 2014.
23. Khalid R, Arshad M, Bilal M, Mehboob F. 2013. Cadmium removal from wastewater using aquatic macrophyte – Nasturtium officinale. ESDEV 2013, CIIT Abbottabad, Pakistan. 25-27 Aug, 2013.
24. Iqbal A, Arshad M. 2013. Hydrocarbon degradation potential of endophytic bacterial isolates from petroleum refinery wastewater". ESDEV 2013, CIIT Abbotabad, Pakistan. 25-27 Aug, 2013.
25. Arshad M. 2012. Biotechnology can contribute to modify lignin contents and simplify biomass to fuel conversion. VASSCAA-6, Renewable Energy Technologies. Pak-China Centre, Islamabad, Pakistan. 11-12 Oct 2012.
26. Arshad M. 2012. Comet Assay: an effective tool for genotoxicity and environmental monitoring. International Conference of Toxicology, UVAS, Lahore, Pakistan. 26-27 Jan 2012.
27. Rashid S, Arshad M, Hashmi I, Baig MA. 2012. Genotoxic impact of airborne pollutants on leucocytes. International Conference of Toxicology, UVAS, Lahore, Pakistan. 26-27 Jan 2012.
28. Arshad M. 2012. Biomass to fuel: Biotechnology can help to reduce lignin contents and simplify extraction procedure. ASTech-2012. CIIT Vehari, Pakistan. 26 Feb 2012.
29. Arshad M. 2011. Genetic engineering for environmental remediation. Environmental Issues & Remedies, University of Agriculture, Faisalabad, Pakistan. 23 Nov 2011.
30. Arshad M. 2010. Biotechnology and renewable energy systems. 1st International Symposium on Vacuum Science and Technology, Islamabad. November 2-6, 2010. 
31. Arshad M, Kallerhoff J, Dumat C, Islam E, Khan QM. 2010. Genetic engineering for the environment: Concepts and applications. 1st National Conference on Biotechnology and Microbiology (FNCOBAM 2010), University of Peshawar, KPK, Pakistan. July 4-7, 2010.
32. Arshad M, Shahid M, Pradere P, Dumat CJ. 2009. Gestion floristique de sols pollués par métaux: étude long terme de la phytoextraction du plomb. 2e rencontre nationale de la recherche sur les sites et sols pollués. Paris, France. 20-21 Oct. 2009.
33. Uzu G, Arshad M, Dumat CJ. 2009. Etude comparative de la remediation d'un sol contaminé au plomb par des techniques de phytoextraction et physicochimique. 2e rencontres nationales de la recherche sur les sites et sols pollués. Paris, France. 20-21 Oct. 2009. 
34. Arshad M, Dumat C, Silvestre J, A. Jauneau, Pinelli E, Kallerhoff J. 2008. Phytoextraction of lead by scented Pelargonium cultivars: phytoavailability, localization and interaction with cations. 4th European Bioremediation Conference, Chania, Crete, Greece. 3-6 Sep, 2008.
35. Arshad M, Silvestre J, Dumat C, Pinelli E, Kallerhoff J.  2008. Optimizationof in vitro plant regeneration for lead hyperaccumulator scented Pelargonium cultivars. 4th European Bioremediation Conference, Chania, Crete, Greece. 3-6 Sep, 2008.
36. Arshad M, Silvestre J, Kallerhoff J, Pinelli E, Kaemmerer M, Pradere P, Shahid M, Dumat C. 2007. Des Pélargoniums odorants pour la phyto-remédiation de sols contaminés par les métaux. Pollutec, Nov, 27-30, 2007. Paris - Nord Villepinte, France.
37. Le Cadre E, Gerard F, Devau N, Clairotte M, Li H, Aznar JL, Arshad M, Jaillard B, Hinsinger P. 2007. Soil pH modifications in the rhizosphere: a real impact on P availability in soil? Rhizopshere 2, International Conference. Montpellier, France. 26-31 Aug, 2007. 
38. Arshad M, Le Cadre E, Hinsinger P, Jaillard B. 2006. Interaction between soil pH buffer capacity and phosphorus bioavailability. 3rd International Symposium:  Phosphorus Dynamics in Soil-Plant Continuum. Uberlândia, Minas Gerais, Brazil. 14-19 May, 2006.

Book Chapters 

1. Imtiaz J, Hashmi I, Saeed A, Qazi IA, Arshad M. 2013. Development of PCR protocol for detection of Escherichia coli in drinking water. In: Water Resources Management VII, edited by C. A. Brebbia, WIT Press, UK. P225-234.
2. Farooq S, Hashmi I, Arshad M, Qazi IA. 2013. Acetanilide herbicide degradation using indigenous soil microorganisms. In: Safety and Security Engineering V, edited by F. Garzia, C. A. Brebbia, M. Guarascio. WIT Press, UK. P685-690.

Industrial/International Collaborations 

1. In collaboration with SCME NUST, we worked on development of Craboxymethyl Cellulose – CMC from Cotton Gin Waste at lab scale. We contributed in different steps and particularly toxicity assessment. For technology transfer to industry in consultation with ST Venture, NUST, a contract with Shafi Res-Chem, Lahore was signed for further research and development.
2. In collaboration with INP-ENSAT, Ecolab, France we are working on development of an integrated strategy for phytoremediation of lead contaminated soils.
3. DxGen, Seoul, South Korea
4. Collaboration with International faculty:
a. Prof. Jean Kallerhoff, Ecolab, France
b. Prof. Hyung – Kyoon Choi, Chung Ang University, Korea 
c. Dr. Tae Jung Park, Associate Professor, Chung Ang University, Korea

Partial Sequences Published at GenBank of NCBI
Partial sequences of 130 bacterial strains with Accession Numbers are published online at NCBI. The sequence numbers are;
03 Sequences Accession Numbers - KM520129 – 520131
07 Sequences Accession Numbers - KP117040 – 117046
11 Sequences Accession Numbers - KX262672 – 262672
109 Sequences Accession Numbers - LC270154 – 270262fcfr

Other Activities 

EBG organized International Conference on Bio-approaches for Environment and Sustainability (22 – 23 February, 2017).