Skip Ribbon Commands
Skip to main content
 
Student Projects
BS Student’s Research Projects
 
Project Title: Antibacterial activity of Lallemantia royleana (Benth.) indigenous to Pakistan
Student Name: Seemab Mahmood
Degree Level: BS Applied Biosciences
Session: 2008-2012
Supervisor: Dr. Muhammad Qasim Hayat
Project Description: Chemical isolates of many medicinal plants have been used as anti-bacterial agents worldwide. Due to increase in antibiotic resistance, it is the need of hour to look for the alternatives. In this research, the antibacterial activity of four extracts (methanol, ethanol, chloroform and aqueous) of L. royleana seeds were evaluated against four bacterial strains (Escherichia. coli, Enterobacter cloaceae, Pseudomonas aeruginosa and Staphylococcus aureus) for the first time by disc diffusion method. All organic extracts of L. royleana seeds displayed significant anti-bacterial activity against all the test bacteria. No antibacterial activity was observed in case of aqueous extracts. The chloroform extract exhibited highest anti-bacterial activity by giving greater values for zone of inhibition for all strains of bacteria. The seed of the plant demonstrated to be most potent against S. aureus infections and gastro-intestinal problems caused by E. coli and E. cloaceae. Results of the study have shown that L. royleana is the paramount candidate for phytochemical studies for the isolation of novel therapeutic compounds.
Status: Completed
 
Project Title: Antibacterial Activity of Artemisia scoparia Waldst & Kit. Collected from Islamabad, Pakistan
Student Name: Hafsa Zafar
Degree Level: BS Applied Biosciences
Session: 2008-2012
Supervisor: Dr. Muhammad Qasim Hayat
Project Description: A large number of the plants are claimed to possess the antibiotic properties in the traditional system and are used extensively worldwide. This study was conducted to investigate the antibacterial potential of Artemisia scoparia (Redstem Wormwood) which is commonly used in folk medicines in Pakistan and worldwide. For this reason, methanol, ethanol, chloroform and aqueous extracts of whole plant of Artemisia scoparia were tested against Vibrio cholera, Acinetobacter junii, Klebsiella pneumonia and Staphylococcus aureus for their antibacterial potential using disc diffusion method. All extracts except aqueous extract exhibited antibacterial activity against all the bacteria used in this study. Highest values of zone of inhibition were observed for ethanol and methanol extracts against Acinetobacter junii and Klebsiella pneumonia while lowest values were recorded for all extracts against Staphylococcus aureus. Results have shown that Artemisia scoparia has potentials for isolation of anti-bacterial compounds. Further studies may lead to characterization of such isolates.
Status: Completed

Project Title: Identification of salt stress responsive gene(s) in Solanum tuberosum. L
Student Name: Bushra Ahmad
Degree Level: BS Applied Biosciences
Session: 2013-2017
Supervisor: Dr. Faiza Munir
Project Description: Pakistan is the seventh largest potato (Solanum tuberosum.L) producer in the world but its yield and quality is greatly affected by abiotic stresses. Salinity is among the major abiotic factors responsible for the reduction in its yield. It has been reported that salinity level of 2.0- 3.0 dS/m can cause up to 50 % yield reduction. The main objective of this study was to locate salt and heat stress responsive genes and to conduct phylogenetic analysis with previous research data. In this study, two commercially valuable varieties of potato (Solanum tuberosum var. Nazca and Solanum tuberosum var. Elbaida) have been studied for salinity. In-silico and in-vitro analysis have been carried out for identification of genes that are responsible for salinity stress. DNA isolation of gene of interest for salinity was amplified via PCR. The amplified product of SAP III gene was around 700bps. The amplified products will be sequenced, translated and phylogenetically analysed. The research data concluded from this study can be used to overcome yield losses by developing salinity resistant varieties of potato and other commercially important crop plants in future.
Status: Completed

Project Title: Identification and analysis of stilbene synthesis pathway genes in Arachis hypogaea L.
Student Name: Rameeza Hassan & Lubna Tauhidi
Degree Level: BS Applied Biosciences
Session: 2012-2015
Supervisor: Dr. Rabia Amir
Project Description: Arachis hypogaea (Peanut Plant) a crop of great significance, belongs to the Fabaceae family (legume family). It is used worldwide as a source of food and holds great importance as an oil bearing plant along with is medicinal properties. Peanut is among one of the major crops being cultivated in Pakistan. Owing to its great importance there is a need to evolve new varieties to further enhance its production. Targeting flavonoids to enhance production of peanut plant is an attractive approach. Flavonoids are secondary metabolites present in all parts of the peanut plant. They impart health benefits through a variety of metabolic cell signaling pathways. In conditions of stress these secondary metabolites are produced providing protection against harsh environmental conditions, acting as antimicrobial and natural pesticide to rescue the plant form any probable damage. In foods, flavonoids provide good taste, color and protect minerals, vitamins, enzymes and oxidation of fat. They also have roles in filtration of UV rays, nitrogen fixation and pigmentation to attract pollinators. They are also being considered for use as pharmaceuticals improving human health. Pertaining to these beneficial attributes flavonoids have great economic value and are an attractive source to enhance production of peanut plant. Among these secondary metabolites are stilbenes that provide potential health benefits against cancer and heart diseases along with other multiple functions similar to those of flavonoids. They are synthesized under conditions of stress like low temperature, pathogen attack and wounding. 
Status: Completed