Virology & Molecular immunology lab focuses on the study of viruses – submicroscopic, parasitic particles of genetic material contained in a protein coat and virus-like agents. Our lab focuses on the following aspects of viruses: their structure, classification and evolution, their ways to infect and exploit host cells for reproduction, their interaction with host organism physiology and immunity, the diseases they cause, the techniques to isolate and culture them, and their use in research and therapy. Virology is considered to be a subfield of microbiology or of medicine. The group is working on the development of intra-genotypic chimeras and construction of consensus cDNA clones for common Pakistani HCV and HIV subtype or Pakistani isolates that can be used for entry and neutralization studies and structural and functional organization of genes. Another aim of the group is to generate a national depository of HCV sequences to understand the extent of divergence and evolution of this virus.
Dr. Sobia Manzoor (firstname.lastname@example.org)
Dr Aneela Javed (email@example.com)
Ms Yasmin Badshah (firstname.lastname@example.org)
HCV lab is fully equipped with the basic & specialized instruments to facilitate research including conventional PCR machines (ABI), Real time PCR, Gel Electrophoresis systems, Gel documentation system, Western Blotting systems (BioRad), Laminar flow safety cabinets, Sonicator, Ependroff Biophotometer, Ultra centrifuge, and -80°C & -150°C (Sanyo, Japan) Freezers etc.
Projects & Grants
• We are currently working on the following funded projects:
• Pak-US Science and Technology Cooperative Program
• Hepatitis C Virus (HCV) Management in Pakistan, in collaboration with Rockefeller University New York, NY, USA
• Higher Education Commission (HEC) Grant, Islamabad
• Development of Cellular model for Pakistani hepatitis C Virus Infection for mechanistic studies and vaccine/drug testing.
• Immunization with Peptide against Hepatitis C Virus (HCV).
Recent Publications from our Group
i. Khalid A, Hussain T, Manzoor S, Saalim M, Khaliq S. PTEN: A potential prognostic marker in virus-induced hepatocellular carcinoma. Tumour Biol. 2017 Jun;39(6):1054.
ii. Khalid M, Brisson L, Tariq M, Hao Y, Guibon R, Fromont G, Mortadza SAS, Mousawi F, Manzoor S, Roger S, Jiang LH. Carcinoma-specific expression of P2Y11 receptor and its contribution in ATP-induced purinergic signalling and cell migration in human hepatocellular carcinoma cells. Oncotarget. 2017 Jun 6; 8(23):37278-37290.
iii. Rauf M, Fatima-Tuz-Zahra, Manzoor S, Mehmood A, Bhatti S. Outbreak of chikungunya in Pakistan. Lancet Infect Dis. 2017 Mar; 17(3):258. Epub 2017 Feb 23.
iv. Bhatti S, Manzoor S. Molecular epidemiology and clinical features of Hepatitis C Virus (HCV) in epidemic areas of Interior Sindh, Pakistan. Pak J Med Sci. 2016 Sep-Oct; 32(5):1279-1282.