HCV persistent infections develop chronic hepatitis, fibrosis and even liver cancer. We are working to check the cellular and molecular effects of HCV non-structural proteins expression on genes involved in mitochondrial mediated apoptosis pathway using cell culture based techniques. We are also developing various HCV sub-genomic replicons which can successfully replicate in cell culture and ELISA based in-vitro infectious assay for Pakistani isolates of HCV. The group is working on the development of intra-genotypic chimeras and construction of consensus cDNA clones for common Pakistani HCV subtype or Pakistani isolates that can be used for entry and neutralization studies and structural and functional organization of genes. Long term non progressors provide us a unique opportunity to explore the natural ways of combating HIV. Research will focus on exploring the immunological responses against HIV and discovery of novel viral inhibitory factors, with special emphasis on T cell responses in long term non-progressors. Other interests include miRNA and genetic studies with reference to HIV.
Dr. Sobia Manzoor (email@example.com)
• Dr Attya Bhatti (firstname.lastname@example.org)
• Dr Aneela Jawad (email@example.com)
• Dr Maria Shabbir (firstname.lastname@example.org)
The lab is fully equipped with the basic & specialized instruments to facilitate research including conventional Laminar flow safety cabinets, Cell counter, CO2 incubator, Ultra centrifuge and -80°C & -150°C (Sanyo, Japan) Freezers etc.
Projects & Grants
• We are currently working on the following funded projects:
• Pak-US Science and Technology Cooperative Program
• Hepatitis C Virus (HCV) Management in Pakistan, in collaboration with Rockefeller University New York, NY, USA
Recent Publications from our Group
i. Dar HA, Mubashir A, Adil M, Farzeen A, Naseer H, Ayub G, Mansoor S, Javed A. Revisiting the AIDS Epidemic in Pakistan: Where We Stand and What We Must Aim for. AIDS Res Hum Retroviruses. 2017 Jul 11: 10.1089.
ii. Dad R, Malik U, Javed A, Minassian BA, Hassan MJ. Structural annotation of Beta-1,4 -N-acetyl galactosaminyltransferase 1 (B4GALNT1) causing Hereditary Spastic Paraplegia 26. Gene. 2017 Aug 30; 626:258-263. Epub 2017 May 20.
iii. Ahmed S, Mahmood Z, Javed A, Hashmi SN, Zerr I, Zafar S, Zahid S. Effect of Metformin on Adult Hippocampal Neurogenesis: Comparison with Donepezil and Links to Cognition. J Mol Neurosci. 2017 May; 62(1):88-98. Epub 2017 Apr 4.
iv. Javed A, Leuchte N, Salinas G, Opitz L, Stahl-Hennig C, Sopper S, Sauermann U. Pre-infection transcript levels of FAM26F in peripheral blood mononuclear cells inform about overall plasma viral load in acute and post-acute phase after simian immunodeficiency virus infection. J Gen Virol. 2016 Dec; 97 (12):3400-3412. Epub 2016 Oct 18.